Fluorescence imaging is a widely popular method of molecule analysis in chemistry, biochemistry, and medicine, thanks to properties such as elevated sensitivity, enhanced specificity, simplicity in comparison to many alternative microscopic techniques, and the versatility to be used in living cells and organisms. Despite the success of using classical optical filters in fluorescence applications, challenges can often arise due to the diverse nature of fluorophores. As each fluorophore has a unique excitation and emission spectrum, different optical filters are required for different applications, which can lead to higher costs. This White Paper looks at linear variable filters (LVFs) as an alternative to more classical filters, and details their advantages compared to homogeneous filters.

White Paper